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Interestingly, we have found that photoinitiator concentration does not affect pattern resolution, so the lowest possible photoinitiator concentration can be used to minimize potential toxicity Utilizing the parameters in the protocol described above, we have achieved 100- m features with an accuracy of 5 m In general, increasing exposure intensity and decreasing the PEG chain length may enhance resolution However, these alterations, if too extreme, can potentially decrease cell viability depending on the cell type utilized In summary, the protocol outlined in this section describes a method for constructing patterned 3-D hydrogel structures containing living mammalian cells This technique can be broadly utilized for the design of implantable tissue constructs, development of de ned 3-D in vitro culture models, and formation of immobilized cellular arrays as cell-based assays for pharmaceutical drug development.

974 An Energy-Efficient Initialization for the k-Channel RN and Unknown n The main purpose of this subsection is to present an energy-efficient initialization protocol for single-hop, k-channel n station radio networks where the number n of stations is not known beforehand Let C(1), C(2), , C(k) denote the k channels available in the RN The idea is to extend protocol in Subsection 973 to take advantage of the k channels Recall that this initialization protocol first runs protocol Approximation to obtain a good approximation of n/log n Once this approximation is available, protocol Energyefficient-initialization is run Recall that by Lemma 76 in the presence of k channels, protocol Approximation terminates, with high probability, in O{[(log n)2/k] + log n} time slots, with no station being awake for more than O(log n) time slots.

Whatever the rationale is, the implication needs to be examined in detail..

We can extend the energy-efficient initialization protocol for single-channel RNs discussed in Subsection 973 to the case of a k channel radio network as follows Recall that we need to initialize each of the groups H(1), H(2), , H(2I+4) This task can be performed as follows: since we have k channels, 2I+4/k groups can be assigned to each channel and be initialized efficiently Since each group H( j) can be initialized in O(I + 4) time slots, all the 2I+4 groups can be initialized in O(I + 4) (2I+4/k) = O(n/k) time slots, with no station being awake no more than O(log n) time slots After that we use the k channel version of the prefix sums protocol discussed in Section 94.

5 .4

In particular, the formation of speci ed 3-D cellular structures represents an important step toward the development of tissue engineering therapies for spatially complex organs, such as the liver Furthermore, several laboratories have demonstrated the incorporation of adhesive ligands [Hern and Hubbell, 1998; Hersel et al, 2003; Shin et al, 2003], degradable motifs [Bryant and Anseth, 2003; Lutolf et al, 2003; Metters et al, 2000; Sawhney et al, 1993; Seliktar et al, 2004], or growth factors [Gobin and West, 2003; Mann et al, 2001b; Seliktar et al, 2004; Zisch et al, 2003] into PEG-based hydrogels, providing a means to regulate cell attachment and spreading, growth, and other biological functions such as matrix deposition..

This takes 2(2I+4/k) + 2 log k 2 < (64n/k log n) + 2 log k time slots, with no station being awake for more than 2 log(2I+4/k) + 2 log k time slots Therefore, we have the following result Theorem 78 Even if the number n of stations is not known in advance, a single-hop, kchannel n station radio network can be initialized, with probability exceeding 1 O(n 183), in O[(n/k) + log n] time slots, with no station being awake for more than O(log n) time slots..

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point that initiated the on-hook action to release the circuits on the access and delivery sides (both PSTN) of the network. The REL and RLC messages are translated into appropriate VoIP call control and signaling messages (e.g., BYE in SIP, Delete-Connection in MGCP, etc., as discussed in 3) to terminate the RTP session between the ingress and egress IP-PSTN MGWs in the IP-based transport network.

Finally, it is noteworthy to mention that adaptive frequency hopping (AFH) spread spectrum, as used in Bluetooth, enhances system resistance to RF interference by avoiding using crowded frequencies in the hopping sequence This sort of adaptive modulation is much easier to implement with FHSS than with DSSS 13112 Direct-Sequence Spread Spectrum DS transmission is an alternative spread-spectrum technique that might be utilized to transmit a narrowband signal over a much wider frequency band The fundamental approach of DS schemes is to cautiously spread the RF energy over a wide frequency band The DS modulation scheme is accomplished by applying a chipping sequence (CS) to the information bit stream A chip is a binary digit sequence employed by the DS system These bits are higher level data while the chip signals are binary numbers used in encoding (transmitter side) and decoding (receiver side) procedures.